Creation of E.COLI producer strains

Getting recombinant proteins in E. coli.
We carry out projects from the design of protein amino acid sequences and gene synthesis to the development of a pilot industrial regulation for the production of pharmacopoeial-quality recombinant protein substance.

Free Gene Optimization
Guaranteed Protein Service
Term from 4 weeks

Possible project structure:

1

Discussion with the customer

Determining the purpose of use
Literature analysis
Suggestion of implementation options
Drafting of TA
Conclusion of an agreement

2

Creation of a producer

Amino acid sequence design (bioinformatics analysis, 3D modeling)
Gene synthesis and creation of an expression construct
Creation of a producer strain
Expression level check

3

Chromatographic Purification

Protein isolation
Refolding (if needed)
Development of chromatographic purification technology

4

Quality control

Determination of protein purity and identity (HPLC, Western blot)
Determination of concentration
Determination of the endotoxins level
Determination of the producer strain proteins and DNA amount

5

Determination of protein activity

Development of a method and determination of the activity of a purified recombinant protein
Preparing a report and sending the protein and producer to the Customer

Examples of obtained recombinant
proteins

The whole range of services:

Chimeric proteins 3D structure modeling
Creation of expression constructs
Obtaining of recombinant protein strain-producer
Optimization of cultivation conditions and expression induction
Production of producer strain biomass in fermenters from 5 l to 100 l
Development and optimization of recombinant protein isolation and purification technology
Development and optimization of recombinant protein refolding technology
Confirmation of the recombinant protein identity by HPLC and Western blot
Development of a method for determining recombinant protein specific activity
Development of quality control methods
Scaling the technology for production, isolation and purification of recombinant protein
Development of laboratory regulations for obtaining recombinant protein
Development of a pilot-industrial technology for obtaining a recombinant protein
Technology transfer to a laboratory or production site
Supply of reagents and equipment, commissioning and installation for the developed production line