Development of Recombinant Protein Expression Strains

/ Development of Recombinant Protein Expression Strains

We produce recombinant proteins in E. coli, managing projects through all necessary stages— from the initial design of the protein amino acid sequence and gene synthesis, to the development of scalable manufacturing processes for recombinant proteins meeting pharmacopoeial quality standards.

Guaranteed Protein Production

Timeline: starting from 4 weeks

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Free gene optimization

Stages of Project Implementation

Customer Consultation

1

Clarifying project goals and intended applications
Literature review and analysis
Proposal of implementation options
Preparation of detailed technical specifications
Conclusion of the contract

Amino acid sequence design (bioinformatic analysis, 3D modeling)
Gene synthesis and construction of gene-expression cassettes
Development of recombinant protein-producing strain
Expression level verification

Creation of Expression Strain

2

3

Protein extraction
Refolding procedures, if necessary
Development of chromatographic purification methods

Chromatographic Purification

Quality Control

Determination of protein purity and identity (HPLC, Western blot)
Measurement of protein concentration
Quantification of endotoxins
Determination of host strain protein and DNA impurities

4

5

Assessment of Protein Activity

Development of assay methods and activity determination of purified recombinant protein
Preparation of a detailed report and shipment of purified protein and developed strain to the customer