+7 (965) 768 36 79

Creation of E.COLI producer strains

Getting recombinant proteins in E. coli.
We carry out projects from the design of protein amino acid sequences and gene synthesis to the development of a pilot industrial regulation for the production of pharmacopoeial-quality recombinant protein substance.
  • Free Gene Optimization
  • Guaranteed Protein Service
  • Term from 4 weeks
Создание штаммов продуцентов E.coli

Possible project structure:

Discussion with the customer
  • Determining the purpose of use
  • Literature analysis
  • Suggestion of implementation options
  • Drafting of TA
  • Conclusion of an agreement
Creation of a producer
  • Amino acid sequence design (bioinformatics analysis, 3D modeling)
  • Gene synthesis and creation of an expression construct
  • Creation of a producer strain
  • Expression level check
Chromatographic Purification
  • Protein isolation
  • Refolding (if needed)
  • Development of chromatographic purification technology
Quality control
  • Determination of protein purity and identity (HPLC, Western blot)
  • Determination of concentration
  • Determination of the endotoxins level
  • Determination of the producer strain proteins and DNA amount
Determination of protein activity
  • Development of a method and determination of the activity of a purified recombinant protein
  • Preparing a report and sending the protein and producer to the Customer

Examples of obtained recombinant


The whole range of services:

  • Chimeric proteins 3D structure modeling
  • Creation of expression constructs
  • Obtaining of recombinant protein strain-producer
  • Optimization of cultivation conditions and expression induction
  • Production of producer strain biomass in fermenters from 5 l to 100 l
  • Development and optimization of recombinant protein isolation and purification technology
  • Development and optimization of recombinant protein refolding technology
  • Confirmation of the recombinant protein identity by HPLC and Western blot
  • Development of a method for determining recombinant protein specific activity
  • Development of quality control methods
  • Scaling the technology for production, isolation and purification of recombinant protein
  • Development of laboratory regulations for obtaining recombinant protein
  • Development of a pilot-industrial technology for obtaining a recombinant protein
  • Technology transfer to a laboratory or production site
  • Supply of reagents and equipment, commissioning and installation for the developed production line
199178, St. Petersburg,
pr-kt Maly V.O., d.57, building 4,
letter Zh
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